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Last but not least, 1 μCi of 86Rb was included to each tradition and incubation was ongoing at 37 °C for yet another 10 min. Reactions were terminated by washing the cells with ice-cold 100 mM MgCl2 and mobile lysates had been geared up for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain ended up subtracte